CoraLite® Plus 647 Anti-Mouse CD1d (1B1)

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  FC experiment of mouse splenocytes using CL647-65242

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 405 Anti-Mouse CD45R, and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD1d (1B1) (CL647-65242, Clone: 1B1) or CoraLite® Plus 647 Rat IgG2b Isotype Control (LTF-2) (CL647-65211, Clone: LTF-2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL647-65242

  1x10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD1d (1B1) (CL647-65242, Clone: 1B1) (red) or CoraLite® Plus 647 Rat IgG2b Isotype Control (LTF-2) (CL647-65211, Clone: LTF-2) (blue). Cells were not fixed.

CD1d Monoclonal Antibody for FC

Cat No. CL647-65242

Clone No.1B1

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Synonyms

1B1, AI747460, CD1.1, Cd1d1, CD1d1 antigen

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65242 targets CD1d in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Mouse CD1d cDNA-transfected RMA-S mouse T lymphoma

• Full Name: CD1d1 antigen
• Gene Symbol: CD1d
• Gene ID (NCBI): 12479
• RRID: AB_2935024
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P11609
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines.