CD1e Recombinant monoclonal antibody

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  WB analysis of Jurkat using 87398-1-RR

  Jurkat cells were subjected to SDS PAGE followed by western blot with 87398-1-RR (CD1e antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 87398-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87398-1-RR (CD1e antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 87398-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD1e antibody (87398-1-RR, Clone: 252400H8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity of 87398-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87398-1-RR against Human CD1e were performed. The affinity constant is 2.15 nM.

CD1e Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87398-1-RR

Clone No.252400H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

hCD1e, R2, R2G1

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Tested Applications

• Positive WB detected in: Jurkat cells, MOLT-4 cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87398-1-RR targets CD1e in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg9185

  Product name: Recombinant human B2M-CD1E protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-Twin-Strep-Tag

  Domain: 10011182 of

  Sequence: \

• Full Name: CD1e
• Observed Molecular Weight: 27 kDa, 30-45 kDa
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15812
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD1e molecule (CD1e, also known as R2 and CD1A) is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin (PMID: 10948205). The discoverers of the CD1 locus originally separated CD1 proteins into group 1 (CD1a, CD1b, and CD1c) and group 2 (CD1d), based on amino acid sequence homology (PMID: 2467814). CD1e is an important modulator of group 1 and group 2 CD1-restricted responses influencing the lipid antigen availability and the generation and persistence of CD1-lipid complexes (PMID: 21844346). CD1e may exhibit varying degrees of molecular weight migration in immunoblotting due to differences in glycosylation modification levels. The molecular weight of non N-glycosylated CD1e is 27 kDa (PMID: 19196239).