Anti-Human CD2 (TS1/8)

• 

  FC experiment of human PBMCs using 65192-1-Ig

  1X10^6 human PBMCs were surface co-stained with FITC anti-Human CD3 and 0.2 ug Anti-Human CD2 (65192-1-Ig, Clone:TS1/8) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65192-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD2 (65192-1-Ig, Clone:TS1/8) labeled with FlexAble CoraLite® Plus 647 Antibody Labeling Kit for Mouse IgG1 (KFA023) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CD2 Monoclonal Antibody for FC

Cat No. 65192-1-Ig

Clone No.TS1/8

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

CD2, CD2 molecule, Erythrocyte receptor, FLJ46032, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65192-1-Ig targets CD2 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: CD2 molecule
• Calculated Molecular Weight: 351 aa, 39 kDa
• GenBank Accession Number: BC033583
• Gene Symbol: CD2
• Gene ID (NCBI): 914
• ENSEMBL Gene ID: ENSG00000116824
• RRID: AB_2918462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06729
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.