CD2 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 84108-5-RR (same clone as 84108-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84108-5-PBS in a different storage buffer formulation.

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  WB analysis using 84108-5-RR (same clone as 84108-5-PBS)

  NK-92 cells were subjected to SDS PAGE followed by western blot with 84108-5-RR (CD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84108-5-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01045-4

  Sandwich ELISA standard curve of MP01045-4, Human CD2 Recombinant Matched Antibody Pair - PBS only. 84108-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1271. 84108-3-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

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  Sample test of MP01045-4

  Serum of six individual healthy human donors was measured. The human CD2 concentration of detected samples was determined to be 167.0 pg/mL with a range of 119.9 - 223.9 pg/mL.

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  Sample test of MP01045-4

  Jurkat and Molt-4 cell lysate were measured. The human CD2 concentration of detected samples was determined to be 8,318.1 pg/mL (based on a 1.0 mg/mL extract load) in Jurkat cell lysate and 679.3 pg/mL (based on a 4.5 mg/mL extract load) in Molt-4 cell lysate.

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  IF Staining of Jurkat using 84108-5-RR (same clone as 84108-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat (positive) Raji (negative) cells using CD2 antibody (84108-5-RR, Clone: 241222B8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84108-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84108-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84108-5-RR against Human CD2 were performed. The affinity constant is 0.494 nM.

CD2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84108-5-PBS

Clone No.241222B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241222B8, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor

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Product Information

84108-5-PBS targets CD2 as part of a matched antibody pair:

MP01045-4: 84108-5-PBS capture and 84108-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1271

  Product name: Recombinant Human CD2 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 25-209 aa of BC033583

  Sequence: KEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD

• Full Name: CD2 molecule
• Calculated Molecular Weight: 351 aa, 39 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC033583
• Gene Symbol: CD2
• Gene ID (NCBI): 914
• ENSEMBL Gene ID: ENSG00000116824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06729
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.