Mouse CD2 Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis using 84360-4-RR (same clone as 84360-4-PBS)

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 84360-4-RR (CD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01249-1

  Sandwich ELISA standard curve of MP01249-1, Mouse CD2 Recombinant Matched Antibody Pair - PBS only. 84360-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1403. 84360-4-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

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  Sample test of MP01249-1

  The mean CD2 concentration was determined to be 3.0 ng/mL in CTLL-2 cell extract based on a 0.9 mg/mL extract load.

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  IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 84360-4-RR (same clone as 84360-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 84360-4-RR (CD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84360-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01249-1

  Cytometric bead array standard curve of MP01249-1, MOUSE CD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84360-5-PBS. Detection antibody: 84360-4-PBS. Standard: Eg1403. Range: 0.313-40 ng/mL.

CD2 Uni-rAb^® Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84360-4-PBS

Clone No.241548D7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

T-cell surface antigen CD2, Lymphocyte antigen 37, Ly-37, LFA-3 receptor, LFA-2

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Product Information

84360-4-PBS targets CD2 as part of a matched antibody pair:

MP01249-1: 84360-5-PBS capture and 84360-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  FusionProtein

• Full Name: CD2 antigen
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: NM_013486.2
• Gene Symbol: CD2
• Gene ID (NCBI): 12481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08920
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.