CoraLite® Plus 488-conjugated CD2 Recombinant monoclonal antibody

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  IF Staining of Jurkat using CL488-84108-5

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 CD2 antibody (CL488-84108-5, Clone: 241222B8 ) at dilution of 1:200.

CD2 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-84108-5

Clone No.241222B8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

241222B8, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor

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Tested Applications

• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84108-5 targets CD2 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1271

  Product name: Recombinant Human CD2 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 25-209 aa of BC033583

  Sequence: KEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD

• Full Name: CD2 molecule
• Calculated Molecular Weight: 351 aa, 39 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC033583
• Gene Symbol: CD2
• Gene ID (NCBI): 914
• ENSEMBL Gene ID: ENSG00000116824
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06729
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.