Anti-Human CD20 (2H7)

CD20 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

2H7

Cat no : 65085-1-Ig

Synonyms

B lymphocyte antigen CD20, B1, Bp35, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

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Validation Data Gallery

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  FC experiment of human PBMCs using 65085-1-Ig

  1X10^6 human PBMCs were surface co-stained with CL405 Anti-Human CD19 and 0.5 ug Anti-Human CD20 (65085-1-Ig, Clone: 2H7) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or Isotype Control. Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using 65085-1-Ig

  1X10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD73 (65162-1-Ig, Clone:AD2) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65085-1-Ig targets CD20 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human tonsillar B cells
• Full Name: membrane-spanning 4-domains, subfamily A, member 1
• Calculated Molecular Weight: 297 aa, 33 kDa
• GenBank Accession Number: BC002807
• Gene Symbol: CD20
• Gene ID (NCBI): 931
• RRID: AB_2918388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein belonging to the membrane-spanning 4A family (PMID: 3260267; 16785532). CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells (PMID: 7524522). CD20 functions as calcium-permeable cation channel (PMID: 7684739). It is involved in the regulation of B-cell activation, proliferation and differentiation (PMID: 7524522).