Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65162-1-Ig targets CD73 in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Pre-B leukemia cell line Predict reactive species |
| Full Name | 5'-nucleotidase, ecto (CD73) |
| Calculated Molecular Weight | 29 kDa, 63 kDa |
| GenBank Accession Number | BC015940 |
| Gene Symbol | CD73 |
| Gene ID (NCBI) | 4907 |
| RRID | AB_2881479 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P21589 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD73 antibody 65162-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Human CD73 (AD2), catalog number 65162-1-Ig, has 6 cited publications. Citations appear in Advanced Materials, Advanced Healthcare Materials, Neurochemistry International, World Journal of Stem Cells, Disease Markers, and Experimental and Therapeutic Medicine. Research fields span periodontal tissue regeneration, diabetic wound healing, spinal cord injury neurochemistry, cardioprotection via stem cell exosomes, abdominal aortic aneurysm pathogenesis, and oral mucosal fibrosis. All cited applications are flow cytometry in human samples.
| Species | Application | Title |
|---|---|---|
Adv Mater Magnetoelectric Coupling Stimulation Modulates Macrophage Reprogramming for Superior Infected Periodontal Tissue Regeneration. | ||
Adv Healthc Mater Antioxidant MOF Nanozyme-Engineered Periodontal Ligament Stem Cells Promote Diabetic Wound Healing. | ||
Neurochem Int Increased spinal adenosine after subacute cervical injury correlates with sustained upregulation of CD39 and CD73 in microglia | ||
World J Stem Cells Hypoxic preconditioned mesenchymal stem cell-derived exosomes alleviate oxidative stress-induced cardiomyocyte apoptosis through miR-486-5p. | ||
Dis Markers Long Noncoding RNA SBF2-AS1 Promotes Abdominal Aortic Aneurysm Formation through the miRNA-520f-3p/SMARCD1 Axis | ||
Exp Ther Med Adipose-derived mesenchymal stem cell exosomes inhibit transforming growth factor-β1-induced collagen synthesis in oral mucosal fibroblasts. |
