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- KD/KO Validated
CD200 Monoclonal antibody
CD200 Monoclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, IHC, IF, FC
Conjugate
Unconjugated
CloneNo.
5F3D6
Cat no : 66282-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | fetal human brain tissue, human spleen tissue |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | U-937 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 2 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
Product Information
66282-1-Ig targets CD200 in WB, IHC, IF, FC applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD200 fusion protein Ag5045 |
Full Name | CD200 molecule |
Calculated molecular weight | 269 aa, 30 kDa |
Observed molecular weight | 40-50 kDa |
GenBank accession number | BC022522 |
Gene symbol | CD200 |
Gene ID (NCBI) | 4345 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD200 antibody 66282-1-Ig | Download protocol |
IHC protocol for CD200 antibody 66282-1-Ig | Download protocol |
FC protocol for CD200 antibody 66282-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancers (Basel) Multiplex Quantitative Analysis of Tumor-Infiltrating Lymphocytes, Cancer-Associated Fibroblasts, and CD200 in Pancreatic Cancer. | ||
Sci Rep CD200-positive cancer associated fibroblasts augment the sensitivity of Epidermal Growth Factor Receptor mutation-positive lung adenocarcinomas to EGFR Tyrosine kinase inhibitors.
| ||
Oncoimmunology CD200 and CD200R1 are differentially expressed and have differential prognostic roles in non-small cell lung cancer.
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