CD200 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CD200 Monoclonal Antibody for WB, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5F3D6

Cat no : 66282-1-PBS

Synonyms

CD200, CD200 molecule, MOX1, MOX2, MRC, OX 2, OX 2 membrane glycoprotein

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Validation Data Gallery

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  WB analysis of fetal human brain using 66282-1-Ig (same clone as 66282-1-PBS)

  Untreated and PNGase F-treated lysates of fetal human brain tissue and human spleen tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66282-1-Ig (same clone as 66282-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 66282-1-Ig (same clone as 66282-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66282-1-Ig (CD200 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66282-1-Ig (same clone as 66282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66282-1-Ig (CD200 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U-937 using 66282-1-Ig (same clone as 66282-1-PBS)

  1X10^6 U-937 cells were stained with 0.2 ug Anti-Human CD200 (66282-1-Ig, Clone: 5F3D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution of 1:1000 (green), or with 0.2 ug mouse IgG1 isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution of 1:1000 (black). Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 66282-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66282-1-PBS targets CD200 in WB, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD200 fusion protein Ag5045
• Full Name: CD200 molecule
• Calculated Molecular Weight: 269 aa, 30 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC022522
• Gene Symbol: CD200
• Gene ID (NCBI): 4345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).