FcZero-rAb® CoraLite® Plus 647 Anti-Human CD200 Rabbit Recombinant Antibody

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• FcZero-rAb® CoraLite® Plus 647 Anti-Human CD200 Rabbit Recombinant Antibody (CL647-FcA98091-2)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD200 FcZero-rAb^® Recombinant Antibody for FC

Cat No. CL647-FcA98091-2 Copied!

Clone No. 241329G4

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD200 molecule, MOX1, MOX2, MRC, OX 2

• 

  FC experiment of human PBMCs using CL647-FcA98091-2

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 555 Anti-Human CD19 (4G7), and 5 ul CoraLite® Plus 647 Anti-Human CD200 Rabbit RecAb (CL647-FcA98091-2, Clone: 241329G4) or FcZero-rAb® CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-FcA98136, Clone: 240953C9). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using CL647-FcA98091-2

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD200 Rabbit RecAb (CL647-FcA98091-2, Clone: 241329G4) (red) or FcZero-rAb® CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-FcA98136, Clone: 240953C9)(blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-FcA98091-2 targets CD200 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: CD200 molecule
• Calculated Molecular Weight: 269 aa, 30 kDa
• GenBank Accession Number: BC022522
• Gene Symbol: CD200
• Gene ID (NCBI): 4345
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P41217
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).