Tested Applications
| Positive IHC detected in | human skin squamous cell cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human skin cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:10000-1:40000 |
| Immunofluorescence (IF)-P | IF-P : 1:10000-1:40000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67788-1-Ig targets CD207/Langerin in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2479 Product name: Recombinant human CD207 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-328 aa of BC022278 Sequence: MTVEKEAPDAHFTVDKQNISLWPREPPPKSGPSLVPGKTPTVRAALICLTLVLVASVLLQAVLYPRFMGTISDVKTNVQLLKGRVDNISTLDSEIKKNSDGMEAAGVQIQMVNESLGYVRSQFLKLKTSVEKANAQIQILTRSWEEVSTLNAQIPELKSDLEKASALNTKIRALQGSLENMSKLLKRQNDILQVVSQGWKYFKGNFYYFSLIPKTWYSAEQFCVSRNSHLTSVTSESEQEFLYKTAGGLIYWIGLTKAGMEGDWSWVDDTPFNKVQSARFWIPGEPNNAGNNEHCGNIKAPSLQAWNDAPCDKTFLFICKRPYVPSEP Predict reactive species |
| Full Name | CD207 molecule, langerin |
| Calculated Molecular Weight | 328 aa, 37 kDa |
| GenBank Accession Number | BC022278 |
| Gene Symbol | CD207 |
| Gene ID (NCBI) | 50489 |
| RRID | AB_2918552 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UJ71 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD207 protein (also known as langerin protein) is encoded by the CD207 gene and is a type of C-type lectin receptor that is specifically expressed on the surface of LCs. It is one of the specific immunohistochemical markers of LCs (PMID: 37383183, PMID: 28109175). CD207 plays an important role in a series of non-classical antigen processing processes, such as forming Birbeck granules and processing and transporting them to local lymphoid tissue, and inducing LC maturation (PMID: 32326440).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD207/Langerin antibody 67788-1-Ig | Download protocol |
| IHC protocol for CD207/Langerin antibody 67788-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD207/Langerin Monoclonal antibody (1H3A1), catalog number 67788-1-Ig, has 3 total citations. These appear in Bioengineering & Translational Medicine, Naunyn-Schmiedeberg's Archives of Pharmacology, and eLife. The research fields span human skin and Langerhans cell biology (airborne particulate pollution effects), pregnancy-associated genital warts treatment (biocompatible hydrogel), and cryo-electron tomography of Birbeck granules revealing langerin lattice formation mechanisms. Applications cited include IF, IHC, and WB.
| Species | Application | Title |
|---|---|---|
Bioeng Transl Med Assessing the impact of airborne particulate pollution on human skin utilizing a novel human skin equivalent containing MUTZ-3-derived Langerhans cells | ||
Naunyn Schmiedebergs Arch Pharmacol Biocompatible hydrogel eradicates pregnancy-associated genital warts. | ||
Elife Cryo-electron tomography of Birbeck granules reveals the molecular mechanism of langerin lattice formation. |
Reviews
What customers say
Users report that this antibody performs well for immunohistochemistry on human skin tissue, with one reviewer achieving strong results using a Leica Bond RXm platform with Red Chromogen Kit and ER2 antigen retrieval at 100°C for 30 minutes at a 1:2000 dilution. However, another user noted that while IHC staining was satisfactory at 1:500, immunofluorescence applications produced high background staining that reduced signal specificity. Overall, the antibody is considered reliable for IHC on skin samples but may require optimization for IF use.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rafailia (Verified Customer) (04-22-2026) | The antibody performs well in immunohistochemistry (IHC) on human skin tissue. However, it did not yield comparable results in immunofluorescence (IF), likely due to high background staining that reduced signal specificity.
|
Federica (Verified Customer) (11-10-2023) | Stainig done with Leica Bond RXm, Red Chromogen Kit, Antigen Retrival ER2 100C for 30min
 |
