1x10^6 unstreated or GM-CSF, IL-4 and TGF-β treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD207 Rabbit RecAb (98368-2-RR, Clone:242303C1) and PE-Conjugated Donkey Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD1a (HI149) (APC-65172, Clone: HI149). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 unstreated or GM-CSF, IL-4 and TGF-β treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD207 Rabbit RecAb (98368-2-RR, Clone:242303C1) and PE-Conjugated Donkey Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD1a (HI149) (APC-65172, Clone: HI149). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98368-2-RR targets CD207 in FC (Intra) applications and shows reactivity with human samples.
1x10^6 unstreated or GM-CSF, IL-4 and TGF-β treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD207 Rabbit RecAb (98368-2-RR, Clone:242303C1) and PE-Conjugated Donkey Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD1a (HI149) (APC-65172, Clone: HI149). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD207 Recombinant antibody
Catalog Number
98368-2-RR
Citations
-
Dilutions
FC (INTRA) : 0.25 ug per 10^6 cells in 100 μl suspension
Applications
FC (Intra)
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
