Anti-Human CD21 (BU32) Mouse IgG1 Recombinant Antibody

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  FC experiment of human PBMCs using 65512-1-MR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-MR, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65512-1-MR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-MR, Clone:BU32) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65512-1-MR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD21 Mouse Recombinant Antibody (65512-1-MR, Clone:BU32) (left) or 0.1 ug Anti-Human CD21 Mouse Monoclonal Antibody (Clone:BU32) (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488-Conjugated Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

CD21 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65512-1-MR

Clone No.BU32

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC

Synonyms

BU32, C3DR, Complement C3d receptor, Complement receptor type 2, CR2

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65512-1-MR targets CD21 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen:

  N/A

• Full Name: complement component (3d/Epstein Barr virus) receptor 2
• Calculated Molecular Weight: 1092 aa, 119 kDa
• GenBank Accession Number: BC136394
• Gene Symbol: CD21
• Gene ID (NCBI): 1380
• ENSEMBL Gene ID: ENSG00000117322
• RRID: AB_3670299
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P20023
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.