Atlantic Blue™ Anti-Human CD21 (BU32)

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  FC experiment of human PBMCs using AB-65198

  1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD19 and 5 ul Atlantic Blue™ Anti-Human CD21 (AB-65198, Clone:BU32) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using AB-65198

  1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD21 (AB-65198, Clone:BU32) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

CD21 Monoclonal Antibody for FC

Cat No. AB-65198

Clone No.BU32

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

BU32, C3DR, Complement C3d receptor, Complement receptor type 2, CR2

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

AB-65198 targets CD21 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: complement component (3d/Epstein Barr virus) receptor 2
• Calculated Molecular Weight: 1092 aa, 119 kDa
• GenBank Accession Number: BC136394
• Gene Symbol: CD21
• Gene ID (NCBI): 1380
• ENSEMBL Gene ID: ENSG00000117322
• RRID: AB_2935193
• Conjugate: Atlantic Blue™ Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 404 nm / 458 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P20023
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.