Anti-Human CD226 (11A8) Mouse IgG2a Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 65573-1-MR (same clone as 65573-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD226 Mouse Recombinant Antibody (65573-1-MR, Clone: 11A8) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Cells were co-stained with CoraLite® Plus 405 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65573-1-PBS in a different storage buffer formulation.

CD226 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65573-1-PBS

Clone No.11A8

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

DNAM-1, DNAM1, DNAM 1, CD226 molecule, CD226 antigen

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Product Information

65573-1-PBS targets CD226 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Human NK Cells

• Full Name: CD226 molecule
• Calculated Molecular Weight: 336 aa, 39 kDa
• GenBank Accession Number: BC074787
• Gene Symbol: CD226
• Gene ID (NCBI): 10666
• ENSEMBL Gene ID: ENSG00000150637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15762
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).