Anti-Mouse CD23 Rabbit Recombinant Antibody

• 

  FC experiment of mouse splenocytes using 98164-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD23 Rabbit Recombinant Antibody (98164-1-RR, Clone: 241385D1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD19. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98164-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD23 Rabbit Recombinant Antibody (98164-1-RR, Clone: 241385D1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98164-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98164-1-RR against Mouse CD23 were performed. The affinity constant is 1.10 nM.

CD23 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98164-1-RR

Clone No.241385D1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

241385D1

---

Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98164-1-RR targets CD23 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: Fc receptor, IgE, low affinity II, alpha polypeptide
• Calculated Molecular Weight: 38kDa
• GenBank Accession Number: EDL21948.1
• Gene Symbol: Fcer2a
• Gene ID (NCBI): 14128
• RRID: AB_3672307
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P20693-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD23, also known as low-affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centers, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells