FC experiment of mouse splenocytes using 98164-1-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD23 Rabbit Recombinant Antibody (98164-1-RR, Clone: 241385D1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD19. Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD23 Rabbit Recombinant Antibody (98164-1-RR, Clone: 241385D1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD19. Cells were not fixed.
FC experiment of mouse splenocytes using 98164-1-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD23 Rabbit Recombinant Antibody (98164-1-RR, Clone: 241385D1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD23 Rabbit Recombinant Antibody (98164-1-RR, Clone: 241385D1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
Affinity and Kinetic Characterization of 98164-1-RR
Biolayer interferometry (BLl) kinetic assays of 98164-1-RR against Mouse CD23 were performed. The affinity constant is 1.10 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98164-1-RR targets CD23 in FC applications and shows reactivity with mouse samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
CD23, also known as low-affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centers, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells
FC experiment of mouse splenocytes using 98164-1-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD23 Rabbit Recombinant Antibody (98164-1-RR, Clone: 241385D1) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD19. Cells were not fixed.
FC experiment of mouse splenocytes using 98164-1-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD23 Rabbit Recombinant Antibody (98164-1-RR, Clone: 241385D1) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
AFFINITY Figures
Affinity and Kinetic Characterization of 98164-1-RR
Biolayer interferometry (BLl) kinetic assays of 98164-1-RR against Mouse CD23 were performed. The affinity constant is 1.10 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
CD23 Recombinant antibody
Catalog Number
98164-1-RR
Citations
-
Dilutions
Applications
FC
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet