CD232/PLXNC1 Recombinant monoclonal antibody

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• CD232/PLXNC1 Recombinant monoclonal antibody (87759-2-RR)

• 

  WB analysis using 87759-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87759-2-RR (CD232/PLXNC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87759-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87759-2-RR (CD232/PLXNC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 87759-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87759-2-RR (CD232/PLXNC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD232/PLXNC1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87759-2-RR

Clone No.252223D4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

CD232, Plexin-C1, Plxnc1, Vespr

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Tested Applications

• Positive WB detected in: Transfected with Mouse CD232/PLXNC1 HEK-293T cells, mouse brain tissue, HEK-293T cells
• Positive IHC detected in: mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87759-2-RR targets CD232/PLXNC1 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6304

  Product name: Recombinant mouse CD232/PLXNC1 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 35-950 aa of NM_018797.3

  Sequence: ADEPVWRSEQAIGAIAASRADGVFVASGSCLDQLDYSLKNRLSRLYRDQAGNCTEPVSLAPPARPRPGSSFSKLLLPYREGATGLEGLLLTGWTFDRGACEVRPLGNLNRSSLRNGTEVVSCHPQGSTAGVVYRASGTDLWYLAVAATYVLPEPETANRCNPAASDRDTAIALKNTEGRSLATQELGRLKLRGSAGSLHFVDAFLWNGSVYFPYYPYNYTSGAATGWPSMARIAQSTEVLFQGQAALDCDHGHPEGRRLLLSSSLVEAVDIWAGVFSAATGEGQERRSPATTALCLFRMSEIQAHARSCSWDFQATEHNCKEGDRPERVQPIASSTLIHSDLTSVYGTVVMNRTVLFLGTGDGQLLKVVLGENLTSNCPEVIYEIKEETPVFYKLVPHPMKNIYIYLTAGKEVRRIPVANCSKRKSCSECLAAADPHCGWCLPLQRCTFQGDCTHAGSFENWLDISSGPKKCPKIQILRSLRERTTVTIVGSISARHSECVVKNADTGKLLCQGRSQLNWTCACNIPSRPSYNVLVVNATFSFPSWNLSERFNFTNCASLKECPACIRSGCAWCKRDKKCIHPFTPCEPSDYERNQELCQVAVEKSPKDSGGGRVKESKRNRTDGAVQVFYIKAIEPQKISTLGKSNVIVTGANFTQASNITMILRGTSTCERDVIRVSHVLNDTHMKFSLPSSRKEMKDVCIQFDGGTCSSAGALSYIALPHCSLIVPATTWISGGQNITIMGRNFDVIDNLIISHELKGNANVNINVSEYCAATFCRFLAPNLKSSKVRTNVAVKLRVQDTYLDCGTLQYLEDPRFTGYRVESEIDTELEVKIQKENDNFNISKDDIDITLFHGENKQFNCSFENITRNQDLTTILCKIKSIKNANTIASSSKKVRVKLGNLELYVEQESVPST

• Full Name: plexin C1
• Calculated Molecular Weight: 176 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: NM_018797.3
• Gene Symbol: Plxnc1
• Gene ID (NCBI): 54712
• RRID: AB_3745737
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9QZC2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PlxnC1 in mouse encodes a semaphorin receptor that integrates extracellular guidance and immune cues through R-Ras/Rap1-GTPase regulation. Expressed in neurons, epithelial cells, and immune cells, it governs axon targeting, olfactory circuit formation, and inflammatory responses. Loss of PlxnC1 disrupts olfactory axon sorting and modifies immune signaling, underscoring its conserved role in neurodevelopmental patterning and immune homeostasis.