PE Anti-Human Glycophorin A/CD235a (HIR2)

• 

  FC experiment of human red blood cells using PE-65174

  10 ul human whole blood was surface stained with 5 ul PE Anti-Human CD235a (HIR2) (PE-65174) or PE Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

• 

  FC experiment of human red blood cells using PE-65174

  10 ul human whole blood was surface stained with 5 ul PE Anti-Human CD235a (HIR2) (PE-65174) or PE Mouse IgG2b Isotype Control. Cells were not fixed. Red blood cells were gated.

Glycophorin A/CD235a Monoclonal Antibody for FC

Cat No. PE-65174

Clone No.HIR2

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human

Applications

FC

Synonyms

Glycophorin A, CD235a, GpMiIII, GPErik, GPA

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Tested Applications

• Positive FC detected in: human red blood cells

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10 ul cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-65174 targets Glycophorin A/CD235a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Synthetic peptide (Human, N-terminal)

• Full Name: glycophorin A (MNS blood group)
• Calculated Molecular Weight: 150 aa, 16 kDa
• GenBank Accession Number: BC005319
• Gene Symbol: Glycophorin A
• Gene ID (NCBI): 2993
• RRID: AB_2883948
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• UNIPROT ID: P02724
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)