Anti-Human CD235a (HIR2)

CD235a Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG2b

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

HIR2

Cat no : 65174-1-Ig

Synonyms

CD235a, Glycophorin A, GPA, GPErik, GpMiIII, GPSAT, GYPA, HGpMiIII, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MN sialoglycoprotein, MNS, PAS 2, Sialoglycoprotein alpha

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Validation Data Gallery

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  FC experiment of human red blood cells using 65174-1-Ig

  Human red blood cells were surface stained with Anti-Human CD235a (65174-1-Ig, Clone:HIR2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (green), or stained with Mouse IgG2b Isotype Control Antibody and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human red blood cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65174-1-Ig targets CD235a in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / Mouse IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Synthetic peptide (Human, N-terminal)
• Full Name: glycophorin A (MNS blood group)
• Calculated Molecular Weight: 150 aa, 16 kDa
• GenBank Accession Number: BC005319
• Gene Symbol: Glycophorin A
• Gene ID (NCBI): 2993
• RRID: AB_2918449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-A affinity chromatography
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)