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Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL405-98176 targets CD24 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg2104 Product name: Recombinant Mouse CD24 protein (rFc Tag)(HPLC verified) Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 27-53 aa of NM_009846.2 Sequence: NQTSVAPFPGNQNISASPNPSNATTRG Predict reactive species |
| Full Name | CD24a antigen |
| Calculated Molecular Weight | 8 kDa |
| GenBank Accession Number | NM_009846.2 |
| Gene Symbol | CD24 |
| Gene ID (NCBI) | 12484 |
| RRID | AB_3747155 |
| Conjugate | CoraLite® Plus 405 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 399 nm / 422 nm |
| Excitation Laser | Violet Laser (405 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P24807 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 CD24 antibody CL405-98176 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
