CoraLite® Plus 405 Anti-Human CD25/IL-2RA Rabbit Recombinant Antibody

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• CoraLite® Plus 405 Anti-Human CD25/IL-2RA Rabbit Recombinant Antibody (CL405-98469)

CD25/IL-2RA Uni-rAb^® Recombinant Antibody for FC

Cat No. CL405-98469 Copied!

Clone No. 240906E11

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD25, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha, IL2 RA

• 

  FC experiment of human PBMCs using CL405-98469

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with APC Anti-Human CD4 and 5 ul CoraLite® Plus 405 Anti-Human CD25/IL-2RA Rabbit RecAb (CL405-98469, Clone: 240906E11). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using CL405-98469

  1x10^6 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD25/IL-2RA Rabbit RecAb (CL405-98469, Clone: 240906E11) (red) or CoraLite® Plus 405 Rabbit IgG Isotype Control Recombinant Antibody (CL405-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD25/IL-2RA Rabbit RecAb (CL405-98469, Clone: 240906E11) (black, dashed). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL405-98469 targets CD25/IL-2RA in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0408

  Product name: Recombinant Human CD25/IL-2RA protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 22-213 aa of BC156627

  Sequence: ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSC

• Full Name: interleukin 2 receptor, alpha
• Calculated Molecular Weight: 272 aa, 31 kDa
• GenBank Accession Number: BC156627
• Gene Symbol: IL2RA
• Gene ID (NCBI): 3559
• RRID: AB_3747173
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Excitation Laser: Violet Laser (405 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01589
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).