Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody, PBS Only

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  FC experiment of human PBMCs using 65627-1-MR (same clone as 65627-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody (65627-1-MR, Clone:O323) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65627-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65627-1-MR (same clone as 65627-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody (65627-1-MR, Clone: O323) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65627-1-PBS in a different storage buffer formulation.

CD27 Recombinant Antibody for FC

Cat No. 65627-1-PBS

Clone No.O323

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27

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Product Information

65627-1-PBS targets CD27 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: N/A
• Full Name: CD27 molecule
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: BC012160
• Gene Symbol: CD27
• Gene ID (NCBI): 939
• ENSEMBL Gene ID: ENSG00000139193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26842
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.