CoraLite® Plus 647 Anti-Human CD27 Rabbit scFv Antibody

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• CoraLite® Plus 647 Anti-Human CD27 Rabbit scFv Antibody (CL647-SF98346-3)

• Site-specific conjugation

CD27 Recombinant Antibody for FC

Cat No. CL647-SF98346-3

Clone No.242731G8

Host / Type

Rabbit / scFv

Reactivity

human

Applications

FC

Synonyms

CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27

• 

  FC experiment of human PBMCs using CL647-SF98346-3

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 (OKT3), and 0.25 ug CoraLite® Plus 647 Anti-Human CD27 Rabbit scFv Antibody (CL647-SF98346-3, Clone: 242731G8) or 0.25 ug CoraLite® Plus 647 Rabbit scFv Isotype Control (CL647-SF98136, Clone: 240953C9). Cells were not fixed. Lymphocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using CL647-SF98346-3

  1x10^6 human PBMCs were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Human CD27 Rabbit scFv Antibody (CL647-SF98346-3, Clone: 242731G8) (red) or 0.25 ug CoraLite® Plus 647 Rabbit scFv Isotype Control (CL647-SF98136, Clone: 240953C9) (blue). Cells were not fixed. Lymphocytes were gated.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-SF98346-3 targets CD27 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Type: Rabbit / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  CatNo: Eg1644

  Product name: Recombinant Human CD27 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 20-191 aa of BC012160

  Sequence: ATPAPKSCPERHYWAQGKLCCQMCEPGTFLVKDCDQHRKAAQCDPCIPGVSFSPDHHTRPHCESCRHCNSGLLVRNCTITANAECACRNGWQCRDKECTECDPLPNPSLTARSSQALSPHPQPTHLPYVSEMLEARTAGHMQTLADFRQLPARTLSTHWPPQRSLCSSDFIR

• Full Name: CD27 molecule
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: BC012160
• Gene Symbol: CD27
• Gene ID (NCBI): 939
• ENSEMBL Gene ID: ENSG00000139193
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Source: Anti-Human CD27 scFv from clone 242731G8, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.