APC Anti-Human CD3 (UCHT1)

CD3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

FC, FC (Intra)

Conjugate

APC Fluorescent Dye

Publications

2

CloneNo.

UCHT1

Cat no : APC-65151

Synonyms

CD3, CD3E, T3E, TCRE

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Validation Data Gallery

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  FC experiment of human PBMCs using APC-65151

  1X10^6 human PBMCs were surface co-stained with CL488 Anti-Human CD19 and 5 ul APC Anti-Human CD3 (APC-65151, Clone:UCHT1) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using APC-65151

  1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD3 (APC-65151, Clone:UCHT1) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

APC-65151 targets CD3 in FC, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD3e molecule, epsilon (CD3-TCR complex)
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC049847
• Gene Symbol: CD3
• Gene ID (NCBI): 916
• RRID: AB_2883030
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
	FC	Clin Exp Med; An HPK1 inhibitor enhanced the tumour response to anti-PD-1 immunotherapy in non-Hodgkin's lymphoma; Authors - Lin Yang
human	FC (Intra)	Cardiovasc Res; Senescent CD8+ T cells: a novel risk factor in atrial fibrillation; Authors - Xiang Li