CoraLite®594 Anti-Mouse CD3 (17A2)

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• CoraLite®594 Anti-Mouse CD3 (17A2) (CL594-65077)

CD3 Monoclonal Antibody for FC

Cat No. CL594-65077 Copied!

Clone No. 17A2

Publications (1)

Download datasheet

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Synonyms

Cd3e, 17A2, AI504783, CD3epsilon, CD3ε

• 

  FC experiment of mouse splenocytes using CL594-65077

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD4, and 0.25 ug CoraLite®594 Anti-Mouse CD3 (17A2) (CL594-65077, Clone: 17A2) or CoraLite®594 Rat IgG2b Isotype Control (LTF-2) (CL594-65211, Clone: LTF-2). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of mouse splenocytes using CL594-65077

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite®594 Anti-Mouse CD3 (17A2) (CL594-65077, Clone: 17A2) (red) or CoraLite®594 Rat IgG2b Isotype Control (LTF-2) (CL594-65211, Clone: LTF-2) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65077 targets CD3 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  γδTCR-positive T-T hybridoma D1

• Full Name: CD3 antigen, epsilon polypeptide
• GenBank Accession Number: BC098236
• Gene Symbol: CD3ε
• Gene ID (NCBI): 12501
• RRID: AB_3064914
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Excitation Laser: Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P22646
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

What published studies show

Anti-Mouse CD3 (17A2) (Cat# CL594-65077) has a total of 55 citations. The retrieved citation appears in NPJ Biofilms Microbiomes (2025), a study on Lacticaseibacillus rhamnosus GR-1 preventing autism-like behaviors by reshaping the maternal and offspring microbiome, using flow cytometry (FC) for immune cell analysis. The associated research fields include microbiome-gut-brain axis interactions, neurodevelopmental disorders, and immunology.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	FC	NPJ Biofilms Microbiomes; Lacticaseibacillus rhamnosus GR-1 prevents autism-like behaviors by reshaping the maternal and offspring microbiome; Authors - Ruili Yang