PE-Cyanine7 Anti-Rabbit CD3 Mouse Recombinant Antibody

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• PE-Cyanine7 Anti-Rabbit CD3 Mouse Recombinant Antibody (PY7-98349)

CD3 Uni-rAb^® Recombinant Antibody for FC

Cat No. PY7-98349 Copied!

Clone No. 242568D11

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Host / Isotype

Mouse / IgG2a

Reactivity

rabbit

Applications

FC

Synonyms

CD3 epsilon + CD3 gamma, CD3 epsilon/CD3 gamma, CD3 epsilon, CD3 gamma, CD3e

• 

  FC experiment of rabbit splenocytes using PY7-98349

  1x10^6 rabbit splenocytes were surface stained with FITC Anti-Rabbit CD4, and 0.1 ug PE-Cyanine7 Anti-Rabbit CD3 Mouse RecAb (PY7-98349, Clone: 242568D11) or unstained. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of rabbit splenocytes using PY7-98349

  1x10^6 rabbit splenocytes were surface stained with 0.1 ug PE-Cyanine7 Anti-Rabbit CD3 Mouse RecAb (PY7-98349, Clone: 242568D11) (red) or unstained (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: rabbit splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PY7-98349 targets CD3 in FC applications and shows reactivity with rabbit samples.

• Tested Reactivity: rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3525

  Product name: recombinant rabbit CD3E+CD3G共表达 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007197 of

  Sequence: /

• Full Name: T-cell surface glycoprotein CD3 epsilon chain
• Gene Symbol: CD3E
• Gene ID (NCBI): 100008616
• RRID: AB_3750006
• Conjugate: PE-Cyanine7 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 778 nm
• Excitation Laser: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 epsilon(CD3ε) and CD3 gamma (CD3γ) are transmembrane subunits of the CD3 complex, which is essential for the assembly and surface expression of the T cell receptor (TCR). All of the CD3 subunits carry the so-called immunoreceptor tyrosine-based activation motif (ITAM) in the intracytoplasmic region. The signals are initiated by the specific phosphorylation of two tyrosine residues located in the conserved ITAM (PMID: 21269565).