Anti-Rabbit CD3 Mouse Recombinant Antibody, PBS Only

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• Anti-Rabbit CD3 Mouse Recombinant Antibody, PBS Only (98349-1-PBS)

CD3 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98349-1-PBS Copied!

Clone No. 242568D11

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Host / Isotype

Mouse / IgG2a

Reactivity

rabbit

Applications

FC

Synonyms

CD3 epsilon + CD3 gamma, CD3 epsilon/CD3 gamma, CD3 epsilon, CD3 gamma, CD3e

• 

  FC experiment of rabbit splenocytes using 98349-1-MR (same clone as 98349-1-PBS)

  1x10^6 rabbit splenocytes were surface stained with 0.25 ug Anti-Rabbit CD3 Mouse RecAb (98349-1-MR, Clone:242568D11) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with FITC Anti-Rabbit CD4. Cells were not fixed. This data was developed using the same antibody clone with 98349-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of rabbit splenocytes using 98349-1-MR (same clone as 98349-1-PBS)

  1x10^6 rabbit splenocytes were surface stained with 0.25 ug Anti-Rabbit CD3 Mouse RecAb (98349-1-MR, Clone: 242568D11) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 98349-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 98349-1-MR

  Biolayer interferometry (BLl) kinetic assays of 98349-1-MR against Rabbit CD3E were performed. The affinity constant is 17.3 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

98349-1-PBS targets CD3 in FC applications and shows reactivity with rabbit samples.

• Tested Reactivity: rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3525

  Product name: recombinant rabbit CD3E+CD3G共表达 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10007197 of

  Sequence: /

• Full Name: T-cell surface glycoprotein CD3 epsilon chain
• Gene Symbol: CD3E
• Gene ID (NCBI): 100008616
• RRID: AB_3745999
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD3 epsilon(CD3ε) and CD3 gamma (CD3γ) are transmembrane subunits of the CD3 complex, which is essential for the assembly and surface expression of the T cell receptor (TCR). All of the CD3 subunits carry the so-called immunoreceptor tyrosine-based activation motif (ITAM) in the intracytoplasmic region. The signals are initiated by the specific phosphorylation of two tyrosine residues located in the conserved ITAM (PMID: 21269565).