FcZero-rAb® Biotin Anti-Human CD30/TNFRSF8 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-FcA98124

  1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD30/TNFRSF8 Rabbit RecAb (Biotin-FcA98124, Clone: 241330A12) (red) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD30/TNFRSF8 Rabbit RecAb (Biotin-FcA98124, Clone: 241330A12) (black, dashed) and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

CD30/TNFRSF8 FcZero-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA98124

Clone No.241330A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

CD30, CD30L receptor, D1S166E, Ki 1, Ki 1 antigen

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98124 targets CD30/TNFRSF8 in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: tumor necrosis factor receptor superfamily, member 8
• Calculated Molecular Weight: 64 kDa
• GenBank Accession Number: NM_001243
• Gene Symbol: CD30
• Gene ID (NCBI): 943
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28908
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).