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CD300LF Polyclonal antibody

CD300LF Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13334-1-AP

Synonyms

CMRF35 like molecule 1, CLM-1, CLM1, CLM 1, CD300f

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD300LF Polyclonal antibody (13334-1-AP)

    WB analysis using 13334-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13334-1-AP (CD300LF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using CD300LF Polyclonal antibody (13334-1-AP)

    WB analysis of U-937 using 13334-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 13334-1-AP (CD300LF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using CD300LF Polyclonal antibody (13334-1-AP)

    IP experiment of K-562 using 13334-1-AP

    IP result of anti-CD300LF (IP:13334-1-AP, 4ug; Detection:13334-1-AP 1:1500) with K-562 cells lysate 4000ug.

Tested Applications

Positive WB detected inJurkat cells, U-937 cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13334-1-AP targets CD300LF in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD300LF fusion protein Ag4150
Full Name CD300 molecule-like family member f
Calculated Molecular Weight 290 aa, 32 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC028199
Gene Symbol CD300LF
Gene ID (NCBI) 146722
RRIDAB_2877941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD300LF, also known as CLM1 (CMRF35-like molecule 1), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LF is an inhibitory receptor that has been shown to inhibit various inflammatory processes in myeloid cells, such as macrophages and mast cells.

Protocols

Product Specific Protocols
WB protocol for CD300LF antibody 13334-1-APDownload protocol
IP protocol for CD300LF antibody 13334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

BMC Pulm Med

Identification of new biomarkers for Acute Respiratory Distress Syndrome by expression-based genome-wide association study.

Authors - Dmitry N Grigoryev
View Article