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CD300LG Polyclonal antibody

CD300LG Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15426-1-AP

Synonyms

CMRF35 like molecule 9, CLM-9, CLM9, CLM 9, CD300g

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using CD300LG Polyclonal antibody (15426-1-AP)

    WB analysis of human kidney using 15426-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15426-1-AP (CD300LG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CD300LG Polyclonal antibody (15426-1-AP)

    WB analysis of HepG2 using 15426-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15426-1-AP (CD300LG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CD300LG Polyclonal antibody (15426-1-AP)

    IF Staining of HEK-293 using 15426-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15426-1-AP (CD300LG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman kidney tissue, HepG2 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15426-1-AP targets CD300LG in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD300LG fusion protein Ag8075
Full Name CD300 molecule-like family member g
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC025395
Gene Symbol CD300LG
Gene ID (NCBI) 146894
RRIDAB_2074217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD300LG, also known as CLM9 (CMRF35-like molecule 9), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LG is a receptor which may mediate L-selectin-dependent lymphocyte rollings and may play an important role in inflammation.

Protocols

Product Specific Protocols
WB protocol for CD300LG antibody 15426-1-APDownload protocol
IF protocol for CD300LG antibody 15426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols