Anti-Mouse CD31 (390)

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  IF Staining of bEnd.3 using 65058-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD31 antibody (65058-1-Ig, Clone: 390 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rat IgG(H+L).

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  FC experiment of mouse splenocytes using 65058-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD31 (65058-1-Ig, Clone: 390) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100 (blue). Cells were not fixed.

CD31 Monoclonal Antibody for IF/ICC, FC

Cat No. 65058-1-Ig

Clone No.390

Publications(8)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

IF/ICC, FC

Synonyms

C85791, Cd31, Pecam, PECAM 1, Pecam1

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Tested Applications

• Positive IF/ICC detected in: bEnd.3 cells
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 7 publications below

Product Information

65058-1-Ig targets CD31 in IF/ICC, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C3H/HeJ mouse hematopoietic progenitor cell line 32D
• Full Name: platelet/endothelial cell adhesion molecule 1
• GenBank Accession Number: BC008519
• Gene Symbol: CD31
• Gene ID (NCBI): 18613
• RRID: AB_2918366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q08481
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
	IF	mBio; Human ApoE2 protects mice against Plasmodium berghei ANKA experimental cerebral malaria; Authors - Rui Liang
mouse	IF	J Ethnopharmacol; Investigation of the efficacy of Dengzhan Shengmai capsule against heart failure with preserved ejection fraction; Authors - Ziyi Kang
mouse	IF	J Ethnopharmacol; Berberis dictyophylla F. inhibits angiogenesis and apoptosis of diabetic retinopathy via suppressing HIF-1α/VEGF/DLL-4/Notch-1 pathway.; Authors - Xiaopeng Ai
mouse	IF	Eur J Pharmacol; Crosstalk between endothelial cells with a non-canonical EndoMT phenotype and cardiomyocytes/fibroblasts via IGFBP5 aggravates TAC-induced cardiac dysfunction; Authors - Yue Li
		Phytother Res; A new look at angiogenesis inhibition of geniposide in experimental arthritis by blocking angiopoietin-2 exocytosis; Authors - Peirong Gan
mouse	IF	Adv Mater; Photothermal and Photocatalytic Glycol Chitosan and Polydopamine-Grafted Oxygen Vacancy Bismuth Oxyiodide (BiO1-x I) Nanoparticles for the Diagnosis and Targeted Therapy of Diabetic Wounds; Authors - Jianglong Kong