PE Anti-Mouse CD31 (390)

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  FC experiment of mouse bone marrow cells using PE-65058

  1x10^6 mouse bone marrow cells were surface stained with 0.1 ug PE Anti-Mouse CD31 (PE-65058, Clone: 390) or 0.1 ug PE Rat IgG2a Isotype Control (2A3) (PE-65209, Clone: 2A3). Cells were co-stained with APC Anti-Mouse CD45R (B220) (APC-65139, Clone: RA3-6B2). Cells were not fixed.

CD31 Monoclonal Antibody for FC

Cat No. PE-65058

Clone No.390

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Synonyms

Pecam1, PECAM 1, Pecam, 390

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-65058 targets CD31 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  C3H/HeJ mouse hematopoietic progenitor cell line 32D

• Full Name: platelet/endothelial cell adhesion molecule 1
• GenBank Accession Number: BC008519
• Gene Symbol: CD31
• Gene ID (NCBI): 18613
• RRID: AB_2883855
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q08481
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).