FCGR2A / CD32a Monoclonal antibody

FCGR2A / CD32a Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b









Cat no : 66529-1-Ig


CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a

Tested Applications

Positive WB detected inTF-1 cells, Raji cells, U-937 cells, Mo7e cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inTHP-1 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66529-1-Ig targets FCGR2A / CD32a in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FCGR2A / CD32a fusion protein Ag25878
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC020823
Gene Symbol FCGR2A
Gene ID (NCBI) 2212
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.


Product Specific Protocols
WB protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
IHC protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
IF protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
Standard Protocols
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