FCGR2A / CD32a Monoclonal antibody
FCGR2A / CD32a Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1D3F1
Cat no : 66529-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-937 cells, Raji cells, THP-1 cells, K-562 cells |
Positive IHC detected in | human liver cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | THP-1 cells |
Positive FC detected in | THP-1 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Product Information
66529-1-Ig targets FCGR2A / CD32a in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | FCGR2A / CD32a fusion protein Ag25878 |
Full Name | Fc fragment of IgG, low affinity IIa, receptor (CD32) |
Calculated molecular weight | 35 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | BC020823 |
Gene symbol | FCGR2A |
Gene ID (NCBI) | 2212 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.
Protocols
Product Specific Protocols | |
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WB protocol for FCGR2A / CD32a antibody 66529-1-Ig | Download protocol |
IHC protocol for FCGR2A / CD32a antibody 66529-1-Ig | Download protocol |
IF protocol for FCGR2A / CD32a antibody 66529-1-Ig | Download protocol |
FC protocol for FCGR2A / CD32a antibody 66529-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |