FCGR2A / CD32a Monoclonal antibody

FCGR2A / CD32a Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3F1

Cat no : 66529-1-Ig

Synonyms

CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a



Tested Applications

Positive WB detected inU-937 cells, Raji cells, THP-1 cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inTHP-1 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Sample-dependent, check data in validation data gallery

Product Information

66529-1-Ig targets FCGR2A / CD32a in WB, IHC, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FCGR2A / CD32a fusion protein Ag25878
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Calculated molecular weight 35 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC020823
Gene symbol FCGR2A
Gene ID (NCBI) 2212
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

Protocols

Product Specific Protocols
WB protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
IHC protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
FC protocol for FCGR2A / CD32a antibody 66529-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols