PE Anti-Human CD34 (QBEnd-10)

• 

  FC experiment of human peripheral blood leukocyte using PE-65183

  100 ul human peripheral blood were surface stained with 20.00 ul PE Anti-Human CD34 (PE-65183, Clone: QBEnd-10) and 5 ul APC Anti-Human CD45 (APC-65109, Clone: HI30), and then treated with red blood cell lysis buffer. Cells were not fixed.

CD34 Monoclonal Antibody for FC

Cat No. PE-65183

Clone No.QBEnd-10

Publications(4)

Host / Isotype

Mouse / IgG1

Reactivity

human, non-human primates

Applications

FC

Synonyms

CD34 molecule

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Tested Applications

• Positive FC detected in: human peripheral blood leukocyte

Recommended dilution

• Flow Cytometry (FC): FC : 20 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 4 publications below

Product Information

PE-65183 targets CD34 in FC applications and shows reactivity with human, non-human primates samples.

• Tested Reactivity: human, non-human primates
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human endothelial vesicles

• Full Name: CD34 molecule
• Calculated Molecular Weight: 41 kDa
• GenBank Accession Number: BC039146
• Gene Symbol: CD34
• Gene ID (NCBI): 947
• RRID: AB_2883953
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• UNIPROT ID: P28906
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	FC	Front Cell Dev Biol; Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.; Authors - Xun Xi
Human	FC	Front Pharmacol; CNST is Characteristic of Leukemia Stem Cells and is Associated With Poor Prognosis in AML.; Authors - Haoyu Liu
human	FC	J Endod; Human Dental Pulp Stem Cells Are Subjected to Metabolic Reprogramming and Repressed Proliferation and Migration by the Sympathetic Nervous System via α1B-Adrenergic Receptor; Authors - Chengcan Yang
human	FC	J Dent Sci; N-acetylcysteine promotes cyclic mechanical stress-induced osteogenic differentiation of periodontal ligament stem cells by down-regulating Nrf2 expression.; Authors - Xun Xi