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Tested Applications
| Positive FC detected in | human peripheral blood leukocyte |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 20 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 3 publications below |
Product Information
PE-65183 targets CD34 in FC applications and shows reactivity with Non-human primates, Human samples.
| Tested Reactivity | Non-human primates, Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Human endothelial vesicles Predict reactive species |
| Full Name | CD34 molecule |
| Calculated Molecular Weight | 41 kDa |
| GenBank Accession Number | BC039146 |
| Gene Symbol | CD34 |
| Gene ID (NCBI) | 947 |
| RRID | AB_2883953 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Form | Liquid |
| Purification Method | The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| UNIPROT ID | P28906 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD34 antibody PE-65183 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction. | ||
J Endod Human Dental Pulp Stem Cells Are Subjected to Metabolic Reprogramming and Repressed Proliferation and Migration by the Sympathetic Nervous System via α1B-Adrenergic Receptor | ||
J Dent Sci N-acetylcysteine promotes cyclic mechanical stress-induced osteogenic differentiation of periodontal ligament stem cells by down-regulating Nrf2 expression. |
