PE Anti-Human CD34 (QBEnd-10)

CD34 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1

Reactivity

Non-human primates, Human and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

2

CloneNo.

QBEnd-10

Cat no : PE-65183

Synonyms

CD34, CD34 molecule

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Validation Data Gallery

• 

  FC experiment of human peripheral blood leukocyte using PE-65183

  100 ul human peripheral blood were surface stained with 20.00 ul PE Anti-Human CD34 (PE-65183, Clone: QBEnd-10) and 5 ul APC Anti-Human CD45 (APC-65109, Clone: HI30), and then treated with red blood cell lysis buffer. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood leukocyte

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 20 µl per 10^6 cells in a 100 µl suspension or 20 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Published Applications

• FC: See 2 publications below

Product Information

PE-65183 targets CD34 in FC applications and shows reactivity with Non-human primates, Human samples.

• Tested Reactivity: Non-human primates, Human
• Cited Reactivity: human
• Host / Isotype: Mouse / Mouse IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Human endothelial vesicles
• Full Name: CD34 molecule
• Calculated molecular weight: 41 kDa
• GenBank accession number: BC039146
• Gene symbol: CD34
• Gene ID (NCBI): 947
• Conjugate: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Publications

Species	Application	Title
human	FC	Front Cell Dev Biol; Nrf2 Activation Is Involved in Cyclic Mechanical Stress-Stimulated Osteogenic Differentiation in Periodontal Ligament Stem Cells via PI3K/Akt Signaling and HO1-SOD2 Interaction.; Authors - Xun Xi
human	FC	J Dent Sci; N-acetylcysteine promotes cyclic mechanical stress-induced osteogenic differentiation of periodontal ligament stem cells by down-regulating Nrf2 expression.; Authors - Xun Xi