CoraLite® Plus 555 Anti-Human CD35 (E11)

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  FC experiment of human PBMCs using CL555-65254

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD35 (CL555-65254, Clone:E11) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL555-65254

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human CD35 (CL555-65254, Clone:E11) (red) or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.

CD35 Monoclonal Antibody for FC

Cat No. CL555-65254

Clone No.E11

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

KN, E11, CR1, Complement receptor type 1, C3BR

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-65254 targets CD35 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human CD35 from Acute monocytic leukemia cells and normal blood monocytes

• Full Name: complement component (3b/4b) receptor 1 (Knops blood group)
• Calculated Molecular Weight: 224 kDa
• GenBank Accession Number: NM_000573
• Gene Symbol: CR1
• Gene ID (NCBI): 1378
• ENSEMBL Gene ID: ENSG00000203710
• RRID: AB_3084615
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P17927
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.