Rat CD36 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86246-6-RR (same clone as 86246-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86246-6-RR (CD36/SR-B3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86246-6-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 86246-6-RR (same clone as 86246-6-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86246-6-RR (CD36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86246-6-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 86246-6-RR (same clone as 86246-6-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86246-6-RR (CD36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86246-6-PBS in a different storage buffer formulation.

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  IF Staining of rat liver using 86246-6-RR (same clone as 86246-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD36 antibody (86246-6-RR, Clone: 250237G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86246-6-PBS in a different storage buffer formulation.

CD36 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 86246-6-PBS

Clone No.250237G3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Adipocyte membrane protein, Fat, Fatty acid translocase, Fatty acid transport protein, GP3B

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Product Information

86246-6-PBS targets CD36 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3222

  Product name: Recombinant Rat CD36 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 30-439 aa of

  Sequence: GDLLIEKTIKREVVLEEGTIAFKNWVKTGTTVYRQFWIFDVQNPEEVAKNSSKIKVKQRGPYTYRVRYLAKENITQDPKDSTVSFVQPNGAIFEPSLSVGTENDNFTVLNLAVAAAPHIYTNSFVQGVLNSLIKKSKSSMFQTRSLKELLWGYKDPFLSLVPYPISTTVGVFYPYNNTVDGVYKVFNGKDNISKVAIIDTYKGKRNLSYWESYCDMINGTDAASFPPFVEKSQTLRFFSSDICRSIYAVFESEVNLKGIPVYRFVLPANAFASPLQNPDNHCFCTEKVISNNCTSYGVLDIGKCKEGKPVYISLPHFLHASPDVSEPIEGLNPNEDEHRTYLDVEPITGFTLQFAKRLQVNILVKPARKIEALKNLKRPYIVPILWLNETGTIGDEKAEMFRNQVTGKIK

• Full Name: CD36 molecule (thrombospondin receptor)
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 88 kDa
• Gene Symbol: Cd36
• Gene ID (NCBI): 29184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07969
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD36, also named as GP3B and GP4, is a membrane glycoprotein present on platelets, monocytes, erythroid precursors, endothelial cells, and several tumor cell lines. CD36 binds to collagen, thrombospondin, anionic phospholipids, long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport and oxidized LDL. CD36 may function as a cell adhesion molecule. It mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes.