CD36 Recombinant monoclonal antibody

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  WB analysis using 86246-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86246-6-RR (CD36/SR-B3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat spleen using 86246-6-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86246-6-RR (CD36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 86246-6-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 86246-6-RR (CD36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat liver using 86246-6-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CD36 antibody (86246-6-RR, Clone: 250237G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD36 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 86246-6-RR

Clone No.250237G3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Adipocyte membrane protein, Fat, Fatty acid translocase, Fatty acid transport protein, GP3B

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Tested Applications

• Positive WB detected in: rat heart tissue, mouse heart tissue, rat adipose tissue, mouse adipose tissue, rat spleen tissue
• Positive IHC detected in: rat spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86246-6-RR targets CD36 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3222

  Product name: Recombinant Rat CD36 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 30-439 aa of

  Sequence: GDLLIEKTIKREVVLEEGTIAFKNWVKTGTTVYRQFWIFDVQNPEEVAKNSSKIKVKQRGPYTYRVRYLAKENITQDPKDSTVSFVQPNGAIFEPSLSVGTENDNFTVLNLAVAAAPHIYTNSFVQGVLNSLIKKSKSSMFQTRSLKELLWGYKDPFLSLVPYPISTTVGVFYPYNNTVDGVYKVFNGKDNISKVAIIDTYKGKRNLSYWESYCDMINGTDAASFPPFVEKSQTLRFFSSDICRSIYAVFESEVNLKGIPVYRFVLPANAFASPLQNPDNHCFCTEKVISNNCTSYGVLDIGKCKEGKPVYISLPHFLHASPDVSEPIEGLNPNEDEHRTYLDVEPITGFTLQFAKRLQVNILVKPARKIEALKNLKRPYIVPILWLNETGTIGDEKAEMFRNQVTGKIK

• Full Name: CD36 molecule (thrombospondin receptor)
• Calculated Molecular Weight: 53 kDa
• Observed Molecular Weight: 88 kDa
• Gene Symbol: Cd36
• Gene ID (NCBI): 29184
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07969
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD36, also named as GP3B and GP4, is a membrane glycoprotein present on platelets, monocytes, erythroid precursors, endothelial cells, and several tumor cell lines. CD36 binds to collagen, thrombospondin, anionic phospholipids, long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport and oxidized LDL. CD36 may function as a cell adhesion molecule. It mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes.