CoraLite® Plus 647 Anti-Human CD36 (SMΦ)

CD36 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgM, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

SMΦ

Cat no : CL647-65248

Synonyms

CD36, CHDS7, FAT, Fatty acid translocase, Glycoprotein IIIb, GP3B, GP4, GPIIIB, GPIV, PAS 4, PAS IV, PASIV, Platelet collagen receptor, Platelet glycoprotein 4, Platelet glycoprotein IV, SCARB3, Thrombospondin receptor

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Validation Data Gallery

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  FC experiment of human peripheral blood platelets using CL647-65248

  1X10^6 human peripheral blood platelets were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD36 (CL647-65248, Clone:SMΦ), or CoraLite® Plus 647 Mouse IgM Isotype Control. Cells were not fixed. CD41a positive cells were gated.

• 

  FC experiment of human peripheral blood platelets using CL647-65248

  1X10^6 human peripheral blood platelets were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD36 (CL647-65248, Clone:SMΦ), or CoraLite® Plus 647 Mouse IgM Isotype Control. Cells were not fixed. CD41a positive cells were gated.

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Tested Applications

• Positive FC detected in: human peripheral blood platelets

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65248 targets CD36 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgM, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD36 molecule (thrombospondin receptor)
• Calculated Molecular Weight: 472 aa, 53 kDa
• GenBank Accession Number: BC008406
• Gene Symbol: CD36
• Gene ID (NCBI): 948
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD36, also named as GP3B and GP4, is an 88-kDa membrane glycoprotein present on platelets, monocytes, erythroid precursors, endothelial cells, and several tumor cell lines. CD36 binds to collagen, thrombospondin, anionic phospholipids, long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport and oxidized LDL. CD36 may function as a cell adhesion molecule. It mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutation of CD36 will cause platelet glycoprotein IV deficiency which known as CD36 deficiency. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7).