CoraLite® Plus 647 Anti-Human CD39/ENTPD1 Rabbit scFv Antibody

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• CoraLite® Plus 647 Anti-Human CD39/ENTPD1 Rabbit scFv Antibody (CL647-SF98102)

• Site-specific conjugation

CD39/ENTPD1 Recombinant Antibody for FC

Cat No. CL647-SF98102

Clone No.241292G7

Host / Type

Rabbit / scFv

Reactivity

human

Applications

FC

Synonyms

ENTPD1, ATP diphosphohydrolase, ATPDase, ATP-DPH, CD39

• 

  FC experiment of human PBMCs using CL647-SF98102

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD4, and 0.25 ug CoraLite® Plus 647 Anti-Human CD39/ENTPD1 Rabbit scFv Antibody (CL647-SF98102, Clone: 241292G7) or 0.25 ug CoraLite® Plus 647 Rabbit scFv Isotype Control (CL647-SF98136, Clone: 240953C9). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using CL647-SF98102

  1x10^6 human PBMCs were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Human CD39/ENTPD1 Rabbit scFv Antibody (CL647-SF98102, Clone: 241292G7) (red) or 0.25 ug CoraLite® Plus 647 Rabbit scFv Isotype Control (CL647-SF98136, Clone: 240953C9) (blue). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-SF98102 targets CD39/ENTPD1 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Type: Rabbit / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  Recombinant Protein

• Full Name: ectonucleoside triphosphate diphosphohydrolase 1
• Calculated Molecular Weight: 58 kDa
• GenBank Accession Number: BC047664
• Gene Symbol: CD39
• Gene ID (NCBI): 953
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Source: Anti-Human CD39/ENTPD1 scFv from clone 241292G7, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

ENTPD1 (Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers (PMID: 9733785 ).