Anti-Mouse CD3e Armenian Hamster scFv Antibody

• Home
• Products
• Primary Antibodies
• Anti-Mouse CD3e Armenian Hamster scFv Antibody (SF65651-1-AR)

CD3e Recombinant Antibody for FC

Cat No. SF65651-1-AR Copied!

Clone No. 145-2C11

Download datasheet

Host / Type

Armenian Hamster / scFv

Reactivity

mouse

Applications

FC

Synonyms

145-2C11, AI504783, CD3, CD3epsilon, CD3ε

• 

  FC experiment of mouse splenocytes using SF65651-1-AR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD3e Mouse scFv Antibody (SF65651-1-AR, Clone: 145-2C11) or Rabbit scFv Isotype Control (SF98136-1-RR, Clone: 240953C9), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were then stained with CoraLite® Plus 750 Anti-Mouse CD19 (1D3) (CL750-65290, Clone: 1D3). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of mouse splenocytes using SF65651-1-AR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD3e Mouse scFv Antibody (SF65651-1-AR, Clone: 145-2C11) (red) or Rabbit scFv Isotype Control (SF98136-1-RR, Clone: 240953C9) (blue), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for CD3e in ?

Chat with Able

---

Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

SF65651-1-AR targets CD3e in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Type: Armenian Hamster / scFv
• Class: Recombinant
• Type: Single-chain variable fragment antibody (scFv)
• Immunogen:

  N/A

• Full Name: CD3 antigen, epsilon polypeptide
• GenBank Accession Number: BC098236
• Gene Symbol: CD3ε
• Gene ID (NCBI): 12501
• Conjugate: Unconjugated
• Source: Anti-Mouse CD3e scFv from clone 145-2C11, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His-C tag at the C-terminus.
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: 50mM HEPES, 150mM NaCl, 5mM EDTA, 2mM TCEP, 5% Trehalose, 0.01% Proclin300, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment. 20ul sizes contain 0.1% BSA.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).