Anti-Human CD4 (SK3), PBS Only
CD4 Monoclonal Antibody for Sandwich ELISA, Indirect ELISA
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
Sandwich ELISA, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
SK3
Cat no : 65147-1-PBS
Synonyms
Validation Data Gallery
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
65147-1-PBS targets CD4 as part of a matched antibody pair:
MP50077-1: 65147-1-PBS capture and 68688-1-PBS detection (validated in Sandwich ELISA)
Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Affinity | KD=8.82 x 10-11M KOff=2.00 x 10-5M KOn=2.26 x 105M |
| Immunogen | N/A |
| Full Name | CD4 molecule |
| Calculated Molecular Weight | 55 kDa |
| GenBank Accession Number | BC025782 |
| Gene Symbol | CD4 |
| Gene ID (NCBI) | 920 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
