CD4 Monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP50077-1

  Sandwich ELISA standard curve of MP50077-1, CD4 Monoclonal Matched Antibody Pair - PBS only. 65147-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0333. 68688-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP50077-1

  The mean CD4 concentration was determined to be 5.16 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

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  Sample test of MP50077-1

  Serum of sixteen individual healthy human donors was measured. The human CD4 concentration of detected samples was determined to be 101.4 pg/mL with a range of 12.7 - 255.0 pg/mL.

CD4 Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 68688-1-PBS

Clone No.1E11B4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Product Information

68688-1-PBS targets CD4 as part of a matched antibody pair:

MP50077-1: 65147-1-PBS capture and 68688-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=1.39 x 10^-10M
  K_Off=4.36 x 10^-6M
  K_On=3.13 x 10^4M
• Immunogen:

  CatNo: Eg0333

  Product name: Recombinant Human CD4 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 26-396 aa of BC025782

  Sequence: KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQP

• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• ENSEMBL Gene ID: ENSG00000010610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P01730
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.