Mouse CD4 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86300-3-RR (same clone as 86300-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86300-3-RR (CD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86300-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 86300-3-RR (same clone as 86300-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86300-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 86300-3-RR (same clone as 86300-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86300-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse thymus using 86300-3-RR (same clone as 86300-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86300-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 86300-3-RR (same clone as 86300-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86300-3-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse thymus using 86300-3-RR (same clone as 86300-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86300-3-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse thymus using 86300-3-RR (same clone as 86300-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86300-3-PBS in a different storage buffer formulation.

CD4 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 86300-3-PBS

Clone No.250263G12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

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Product Information

86300-3-PBS targets CD4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3847

  Product name: Recombinant Mouse CD4 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 27-394 aa of NM_013488

  Sequence: KTLVLGKEGESAELPCESSQKKITVFTWKFSDQRKILGQHGKGVLIRGGSPSQFDRFDSKKGAWEKGSFPLIINKLKMEDSQTYICELENRKEEVELWVFKVTFSPGTSLLQGQSLTLTLDSNSKVSNPLTECKHKKGKVVSGSKVLSMSNLRVQDSDFWNCTVTLDQKKNWFGMTLSVLGFQSTAITAYKSEGESAEFSFPLNFAEENGWGELMWKAEKDSFFQPWISFSIKNKEVSVQKSTKDLKLQLKETLPLTLKIPQVSLQFAGSGNLTLTLDKGTLHQEVNLVVMKVAQLNNTLTCEVMGPTSPKMRLTLKQENQEARVSEEQKVVQVVAPETGLWQCLLSEGDKVKMDSRIQVLSRGVNQT

• Full Name: CD4 antigen
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_013488
• Gene Symbol: Cd4
• Gene ID (NCBI): 12504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06332
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).