CoraLite® Plus 488 Anti-Human CD4 (SK3)

CD4 Monoclonal Antibody for FC
Cat No. CL488-65147
Clone No.SK3

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

CD4, CD4 molecule, CD4mut, SK3

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65147 targets CD4 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen

fusion protein

Predict reactive species
Full Name CD4 molecule
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_2883205
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
FormLiquid
Purification MethodAffinity purification
UNIPROT IDP01730
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD4 antibody CL488-65147Download protocol
Standard Protocols
Click here to view our Standard Protocols
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