CoraLite®700 Anti-Human CD4 Rabbit Recombinant Antibody

• 

  FC experiment of human PBMCs using CL700-98042

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3, and 5 ul CoraLite®700 Anti-Human CD4 Rabbit RecAb (CL700-98042, Clone: 240427E12) or unstained. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL700-98042

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®700 Anti-Human CD4 Rabbit RecAb (CL700-98042, Clone: 240427E12) (red) or unstained (blue). Cells were not fixed.

CD4 Uni-rAb^® Recombinant Antibody for FC

Cat No. CL700-98042

Clone No.240427E12

Host / Isotype

Rabbit / IgG

Reactivity

human, non-human primates

Applications

FC

Synonyms

240427E12, CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL700-98042 targets CD4 in FC applications and shows reactivity with human, non-human primates samples.

• Tested Reactivity: human, non-human primates
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0333

  Product name: Recombinant Human CD4 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 26-396 aa of BC025782

  Sequence: KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLAFTVEKLTGSGELWWQAERASSSKSWITFDLKNKEVSVKRVTQDPKLQMGKKLPLHLTLPQALPQYAGSGNLTLALEAKTGKLHQEVNLVVMRATQLQKNLTCEVWGPTSPKLMLSLKLENKEAKVSKREKAVWVLNPEAGMWQCLLSDSGQVLLESNIKVLPTWSTPVQP

• Full Name: CD4 molecule
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: BC025782
• Gene Symbol: CD4
• Gene ID (NCBI): 920
• ENSEMBL Gene ID: ENSG00000010610
• Conjugate: CoraLite®700 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 702 nm / 723 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01730
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).