|Positive FC detected in||human peripheral blood lymphocytes|
|This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl(0.25µg) per 10^6 cells in a 100 µl suspension or 5 µl(0.25µg) per 100 µl of whole blood.|
|Sample-dependent, check data in validation data gallery|
FITC-65134 targets CD4 in FC applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Human peripheral T cells|
|Full Name||CD4 molecule|
|Calculated molecular weight||55 kDa|
|GenBank accession number||BC025782|
|Gene ID (NCBI)||920|
|Conjugate||FITC Fluorescent Dye|
|Excitation/Emission maxima wavelengths||494 nm / 520 nm|
|Purification Method||Affinity purification|
|Storage Buffer||Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.|
|Storage Conditions||Store at 2-8°C. Avoid exposure to light.|
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).