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Anti-Mouse CD40 Rabbit Recombinant Antibody, PBS Only (98122-1-PBS)

Anti-Mouse CD40 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Mouse CD40 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98122-1-PBS-1MG

CD40 Uni-rAb^® Recombinant Antibody for IHC, FC

Cat No. 98122-1-PBS

Clone No. 240874G8

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, FC

Synonyms

240874G8, Bp50, CD40 antigen, Tnfrsf5

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

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Filter: AllIHCFCAffinity

IHC staining of mouse spleen using 98122-1-RR (same clone as 98122-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 98122-1-RR (CD40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98122-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse spleen using 98122-1-RR (same clone as 98122-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 98122-1-RR (CD40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98122-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of mouse splenocytes using 98122-1-RR (same clone as 98122-1-PBS)
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98122-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of mouse splenocytes using 98122-1-RR (same clone as 98122-1-PBS)
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD19 (1D3) (APC-65290, Clone: 1D3). Cells were not fixed. This data was developed using the same antibody clone with 98122-1-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD19 (1D3) (APC-65290, Clone: 1D3). Cells were not fixed. This data was developed using the same antibody clone with 98122-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 98122-1-RR
Biolayer interferometry (BLl) kinetic assays of 98122-1-RR against Mouse CD40 were performed. The affinity constant is 0.997 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information

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Product Information

98122-1-PBS targets CD40 in IHC, FC applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1118 Product name: Recombinant Mouse CD40 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 24-193 aa of NM_011611.2 Sequence: VTCSDKQYLHDGQCCDLCQPGSRLTSHCTALEKTQCHPCDSGEFSAQWNREIRCHQHRHCEPNQGLRVKKEGTAESDTVCTCKEGQHCTSKDCEACAQHTPCIPGFGVMEMATETTDTVCHPCPVGFFSNQSSLFEKCYPWTSCEDKNLEVLQKGTSQTNVICGLKSRMR Predict reactive species
Full Name	CD40 antigen
Calculated Molecular Weight	32 kDa
GenBank Accession Number	NM_011611.2
Gene Symbol	Cd40
Gene ID (NCBI)	21939
RRID	AB_3672269
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P27512-1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).