Tested Applications
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98122-1-RR targets CD40 in IHC, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg1118 Product name: Recombinant Mouse CD40 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 24-193 aa of NM_011611.2 Sequence: VTCSDKQYLHDGQCCDLCQPGSRLTSHCTALEKTQCHPCDSGEFSAQWNREIRCHQHRHCEPNQGLRVKKEGTAESDTVCTCKEGQHCTSKDCEACAQHTPCIPGFGVMEMATETTDTVCHPCPVGFFSNQSSLFEKCYPWTSCEDKNLEVLQKGTSQTNVICGLKSRMR Predict reactive species |
| Full Name | CD40 antigen |
| Calculated Molecular Weight | 32 kDa |
| GenBank Accession Number | NM_011611.2 |
| Gene Symbol | Cd40 |
| Gene ID (NCBI) | 21939 |
| RRID | AB_3672269 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P27512-1 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD40 antibody 98122-1-RR | Download protocol |
| IHC protocol for CD40 antibody 98122-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Mouse CD40 Rabbit Recombinant Antibody (240874G8), catalog number 98122-1-RR, has 1 citation. It appears in the International Journal of Biological Macromolecules, where it was used in flow cytometry to study a novel oral adjuvant—dendritic fibrous nano-silica coated with M cell homing peptide-modified chitosan—evaluating its effects on enhancing both intestinal mucosal and systemic immunity in mice. The associated research field is mucosal immunology and oral vaccine delivery.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol The impact of novel oral adjuvant dendritic fibrous nano-silica coated with M cell homing peptide-modified chitosan on enhancing both the intestinal mucosal and systemic immunity. |
