FITC Anti-Mouse CD40 (1C10)

CD40 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

FITC Fluorescent Dye

Publications

2

CloneNo.

1C10

Cat no : FITC-65062

Synonyms

AI326936, Bp50, Cd40, CD40 antigen, GP39, HIGM1, IGM, IMD3, p50, T BAM, Tnfrsf5

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Validation Data Gallery

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  FC experiment of BALB/c mouse splenocytes using FITC-65062

  1X10^6 BALB/c mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.20 ug FITC-Anti-Mouse CD40 (FITC-65062, Clone: 1C10). Cells were not fixed.

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  FC experiment of BALB/c mouse splenocytes using FITC-65062

  1X10^6 BALB/c mouse splenocytes were surface stained with APC-Anti-Mouse CD3ε (APC-65060, Clone: 145-2C11) and 0.20 ug FITC-Rat IgG2a isotype control antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: BALB/c mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

FITC-65062 targets CD40 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: sCD40
• Full Name: CD40 antigen
• GenBank Accession Number: BC029254
• Gene Symbol: Cd40
• Gene ID (NCBI): 21939
• RRID: AB_2883752
• Conjugate: FITC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 494 nm / 520 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).

Publications

Species	Application	Title
mouse	FC	Int Immunopharmacol; Mesenchymal stem cell-derived extracellular vesicles carrying miR-99b-3p restrain microglial activation and neuropathic pain by stimulating autophagy; Authors - Xu Gao
mouse	FC	Int Immunopharmacol; Huc-MSCs-derived exosomes attenuate neuropathic pain by inhibiting activation of the TLR2/MyD88/NF-κB signaling pathway in the spinal microglia by targeting Rsad2; Authors - Xu Gao